Library Sizes ranged between 129,858,281 and 195,128,478 reads.
Read totals for each library. Duplicated reads are conventionally an high overestimate at this point.
Summary of FastQC flags for each parameter
Heatmap showing mean base qualities for each library
Heatmap showing mean sequence qualities for each library
Heatmap of summed base distributions along each read
GC Content Heatmap normalised to theoretical GC content in the Drerio Transcriptome
GC Content Distributions for all reads showing theoretical GC content from the Drerio Transcriptome
Universal Adapter Content
Total overrepresented sequences for each library
## Overrepresented_sequences missing from 1_KB_A10Aligned.sortedByCoord.out.bam## Overrepresented_sequences missing from 10_KB_B6Aligned.sortedByCoord.out.bam## Overrepresented_sequences missing from 11_KB_B7Aligned.sortedByCoord.out.bam## Overrepresented_sequences missing from 12_KB_B8Aligned.sortedByCoord.out.bam## Overrepresented_sequences missing from 13_KB_B9Aligned.sortedByCoord.out.bam## Overrepresented_sequences missing from 14_KB_C10Aligned.sortedByCoord.out.bam## Overrepresented_sequences missing from 15_KB_C11Aligned.sortedByCoord.out.bam## Overrepresented_sequences missing from 16_KB_C12Aligned.sortedByCoord.out.bam## Overrepresented_sequences missing from 17_KB_C1Aligned.sortedByCoord.out.bam## Overrepresented_sequences missing from 18_KB_C2Aligned.sortedByCoord.out.bam## Overrepresented_sequences missing from 19_KB_C3Aligned.sortedByCoord.out.bam## Overrepresented_sequences missing from 2_KB_A11Aligned.sortedByCoord.out.bam## Overrepresented_sequences missing from 20_KB_C4Aligned.sortedByCoord.out.bam## Overrepresented_sequences missing from 21_KB_C5Aligned.sortedByCoord.out.bam## Overrepresented_sequences missing from 22_KB_C6Aligned.sortedByCoord.out.bam## Overrepresented_sequences missing from 23_KB_C7Aligned.sortedByCoord.out.bam## Overrepresented_sequences missing from 24_KB_C8Aligned.sortedByCoord.out.bam## Overrepresented_sequences missing from 3_KB_A1Aligned.sortedByCoord.out.bam## Overrepresented_sequences missing from 4_KB_A6Aligned.sortedByCoord.out.bam## Overrepresented_sequences missing from 5_KB_A7Aligned.sortedByCoord.out.bam## Overrepresented_sequences missing from 6_KB_B11Aligned.sortedByCoord.out.bam## Overrepresented_sequences missing from 7_KB_B12Aligned.sortedByCoord.out.bam## Overrepresented_sequences missing from 8_KB_B3Aligned.sortedByCoord.out.bam## Overrepresented_sequences missing from 9_KB_B5Aligned.sortedByCoord.out.bam## No overrepresented sequences were detected by FastQCR version 4.0.5 (2021-03-31)
Platform: x86_64-pc-linux-gnu (64-bit)
locale: LC_CTYPE=en_AU.UTF-8, LC_NUMERIC=C, LC_TIME=en_AU.UTF-8, LC_COLLATE=en_AU.UTF-8, LC_MONETARY=en_AU.UTF-8, LC_MESSAGES=en_AU.UTF-8, LC_PAPER=en_AU.UTF-8, LC_NAME=C, LC_ADDRESS=C, LC_TELEPHONE=C, LC_MEASUREMENT=en_AU.UTF-8 and LC_IDENTIFICATION=C
attached base packages: parallel, stats, graphics, grDevices, utils, datasets, methods and base
other attached packages: pander(v.0.6.3), DT(v.0.17), scales(v.1.1.1), readr(v.1.4.0), dplyr(v.1.0.5), stringr(v.1.4.0), magrittr(v.2.0.1), ngsReports(v.1.6.1), tibble(v.3.1.0), ggplot2(v.3.3.3) and BiocGenerics(v.0.36.0)
loaded via a namespace (and not attached): bitops(v.1.0-6), matrixStats(v.0.58.0), lubridate(v.1.7.10), RColorBrewer(v.1.1-2), httr(v.1.4.2), GenomeInfoDb(v.1.26.7), tools(v.4.0.5), bslib(v.0.2.4), utf8(v.1.2.1), R6(v.2.5.0), DBI(v.1.1.1), lazyeval(v.0.2.2), colorspace(v.2.0-0), withr(v.2.4.1), tidyselect(v.1.1.0), compiler(v.4.0.5), Biobase(v.2.50.0), flashClust(v.1.01-2), DelayedArray(v.0.16.3), plotly(v.4.9.3), ggdendro(v.0.1.22), labeling(v.0.4.2), sass(v.0.3.1), digest(v.0.6.27), Rsamtools(v.2.6.0), rmarkdown(v.2.7), XVector(v.0.30.0), jpeg(v.0.1-8.1), pkgconfig(v.2.0.3), htmltools(v.0.5.1.1), MatrixGenerics(v.1.2.1), FactoMineR(v.2.4), htmlwidgets(v.1.5.3), rlang(v.0.4.10), rstudioapi(v.0.13), jquerylib(v.0.1.3), generics(v.0.1.0), farver(v.2.1.0), zoo(v.1.8-9), hwriter(v.1.3.2), jsonlite(v.1.7.2), crosstalk(v.1.1.1), BiocParallel(v.1.24.1), RCurl(v.1.98-1.3), GenomeInfoDbData(v.1.2.4), leaps(v.3.1), Matrix(v.1.3-2), Rcpp(v.1.0.6), munsell(v.0.5.0), S4Vectors(v.0.28.1), fansi(v.0.4.2), lifecycle(v.1.0.0), scatterplot3d(v.0.3-41), stringi(v.1.5.3), yaml(v.2.2.1), MASS(v.7.3-53.1), SummarizedExperiment(v.1.20.0), zlibbioc(v.1.36.0), plyr(v.1.8.6), grid(v.4.0.5), ggrepel(v.0.9.1), forcats(v.0.5.1), crayon(v.1.4.1), lattice(v.0.20-41), Biostrings(v.2.58.0), hms(v.1.0.0), knitr(v.1.31), pillar(v.1.5.1), GenomicRanges(v.1.42.0), reshape2(v.1.4.4), stats4(v.4.0.5), glue(v.1.4.2), packrat(v.0.6.0), evaluate(v.0.14), ShortRead(v.1.48.0), latticeExtra(v.0.6-29), data.table(v.1.14.0), png(v.0.1-7), vctrs(v.0.3.7), gtable(v.0.3.0), purrr(v.0.3.4), tidyr(v.1.1.3), assertthat(v.0.2.1), xfun(v.0.22), viridisLite(v.0.3.0), GenomicAlignments(v.1.26.0), IRanges(v.2.24.1), cluster(v.2.1.1) and ellipsis(v.0.3.1)